Regulatory

Part:BBa_J107100:Design

Designed by: Susanna Zucca   Group: Magni Lab   (2014-12-12)

LuxR-repressible promoter library member


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inspired by the Egland and Greenberg paper ( http://jb.asm.org/cgi/content/abstract/182/3/805 ) and by the Anderson promoter collection sequences.

Source

Oligo annealing.

References

Zucca S, Pasotti L, Politi N, Casanova M, Mazzini G, Cusella De Angelis MG, Magni P: Multi-faceted characterization of a novel LuxR-repressible promoter library for Escherichia coli. ADD LINK